Cover image for Multivariate analysis of fungal volatile metabolites for aflatoxigenic fungi detection [electronic book] / by Dongdi Sun.
Multivariate analysis of fungal volatile metabolites for aflatoxigenic fungi detection [electronic book] / by Dongdi Sun.
Title:
Multivariate analysis of fungal volatile metabolites for aflatoxigenic fungi detection [electronic book] / by Dongdi Sun.
Personal Author:
Publication:
Mississippi State : Mississippi State University, 2015.
Publication Date:
2015
Bibliography Note:
Includes bibliographical references.
Dissertation:
Thesis (Ph.D.) Mississippi State University. Department of Chemistry 2015.
Abstract:
My research focuses on the development of a novel method for the fast detection of aflatoxin-producing fungi from the volatile organic compounds that they produce. Aflatoxins have received great attention because of their demonstrated potent carcinogenic effect in susceptible laboratory animals and their acute toxicological effects in humans. Traditional detection and quantification techniques are considered time-consuming, high cost, and require technical professionals. My research focuses on the development of a novel method for the fast detection of aflatoxin-producing fungi from the volatile organic compounds that they produce. Aflatoxins have received great attention because of their demonstrated potent carcinogenic effect in susceptible laboratory animals and their acute toxicological effects in humans. Traditional detection and quantification techniques are considered time-consuming, high cost, and require technical professionals. To answer these questions, one toxigenic and one nontoxigenic A. flavus isolate were studied to evaluate the microbial volatile organic compound (MVOC) profiles. The results described in chapter two of this dissertation indicate that MVOC production is time-dependent and that aflatoxigenic and non-aflatoxigenic strains have different MVOC expression patterns. Chapter three describes the effects of experimental parameters on fungal volatile metabolites. The identity and quantity of MVOCs can be affected by many factors including SPME fiber type, fungal growth media, and growth temperature. A CAR/PDMS coated fiber performed better than the other SPME fibers by collecting a larger variety and quantity of MVOCs. Fungi grown on the chemical defined liquid media produced much larger quantities of MVOCs compared to the other media. The highest MVOC production results were found at 30°C. The fungi discrimination study was extended in chapter four by including 3 toxigenic and 3 non-toxigenic isolates using multivariate analysis. The results indicate that volatile patterns vary even at the fungal isolate level and that discrimination of aflatoxin-producing fungi from non-toxigenic fungi is possible.
Content Type:
text
Carrier Type:
online resource
Local Note:
Thesis advisor: Todd E. Mlsna.
Language:
English
No. of Holds: